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AKR1D1 Rabbit pAb

Anti-AKR1D1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216967

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216967
Product Name:   Anti-AKR1D1 Rabbit Polyclonal Antibody
Synonyms:   CBAS2; SRD5B1; 3o5bred
UNIPROT ID:   P51857 (Gene Accession – BC130625 )
Background:    The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet.
Immunogen:    Fusion protein of human AKR1D1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216967(AKR1D1 Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216967(Anti-AKR1D1 Antibody) at dilution 1/25.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Mouse liver tissue, A549 cell lysates;
Primary antibody: 216967(AKR1D1 Antibody) at dilution 1/500;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 minutes
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