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AKT Rabbit mAb

Anti-AKT Rabbit Monoclonal Antibody
5/5

$349.00

Cat.#:  N261810

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261810
Product Name:   Anti-AKT Rabbit Monoclonal Antibody
Synonyms:   MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
UNIPROT ID:   Q9Y243
Background:    AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
Immunogen:    A synthetic peptide of human AKT1/2/3
Applications:    WB,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IF: 1/50-1/200
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R08-8F1
MW:    Calculated MW: 56 kDa; Observed MW: 56 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    apoptosis – Bcl-2 family
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of AKT1/2/3(green) in Hela using AKT antibody,and DAPI(blue)

Western blot analysis of AKT in 3T3, rat heart lysates using AKT antibody.

Western blot analysis of AKT1/2/3 in LnCap lysates using AKT antibody.
          Publications