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AKT Rabbit pAb

Anti-AKT Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225657

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225657
Product Name:   Anti-AKT Rabbit pAb
Synonyms:   AKT1
UNIPROT ID:   P31749/P31751/Q9Y243
Background:    Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
Immunogen:    A synthesized peptide derived from human AKT1/2/3
Applications:    WB,IHC-P,ICC/IF,IP,FC
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 56 kDa; Observed MW: 56 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human testis using AKT antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of AKT in K562, A549 and Jurkat lysates using AKT antibody.

Immunofluorescence analysis of AKT in MCF-7 using AKT antibody.
          Publications