Email: info@neweastbio.com   |  Call: 6109452007

AKT Rabbit pAb

Anti-AKT Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N226053

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N226053
Product Name:   Anti-AKT Rabbit pAb
Synonyms:   MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
UNIPROT ID:   Q9Y243
Background:    AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
Immunogen:    Recombinant protein of human AKT3
Applications:    WB,IHC-F,IHC-P,ICC/IF,FC,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 56 kDa; Observed MW: 56 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of Akt (pan) in Jurkat lysates using Akt antibody

Immunocytochemistry analysis of AKT (green) in A549 using AKT antibody,and DAPI(blue).

Western blot analysis of AKT in 3T3, C6 lysates using AKT antibody.

Immunohistochemistry analysis of paraffin-embedded Human tonsil using AKT1/2/3 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
          Publications