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ALDH1A2 Rabbit pAb

Anti-ALDH1A2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220280

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220280
Product Name:   Anti-ALDH1A2 Rabbit Polyclonal Antibody
Synonyms:   RALDH2; RALDH2-T; RALDH(II)
UNIPROT ID:   O94788 (Gene Accession – NP_001193826 )
Background:    This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Four transcript variants encoding distinct isoforms have been identified for this gene.
Immunogen:    Synthetic peptide of human ALDH1A2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Neuroscience, Developmental Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 220280(ALDH1A2 Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the synthetic peptide and then with 220280(Anti-ALDH1A2 Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 220280(Anti-ALDH1A2 Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with synthetic peptide and then with D261255(Anti-ALDH1A2 Antibody) at dilution 1/60.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane: K562 cells;
Primary antibody: 220280(ALDH1A2 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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