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ALDH4A1 Rabbit pAb

Anti-ALDH4A1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216973

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216973
Product Name:   Anti-ALDH4A1 Rabbit Polyclonal Antibody
Synonyms:   P5CD; ALDH4; P5C
UNIPROT ID:   P30038 (Gene Accession – BC007581 )
Background:    This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Immunogen:    Fusion protein of human ALDH4A1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 216973(ALDH4A1 Antibody) at a dilution of 1/35(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 216973(Anti-ALDH4A1 Antibody) at dilution 1/35.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 216973(Anti-ALDH4A1 Antibody) at a dilution of 1/35.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D221569(Anti-ALDH4A1 Antibody) at dilution 1/35.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane: Human fetal liver tissue;
Primary antibody: 216973(ALDH4A1 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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