Email: info@neweastbio.com   |  Call: 6109452007

ANAPC2 Rabbit pAb

Anti-ANAPC2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217013

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217013
Product Name:   Anti-ANAPC2 Rabbit Polyclonal Antibody
Synonyms:   APC2; RP11-350O14.5
UNIPROT ID:   Q9UJX6 (Gene Accession – BC032503 )
Background:    A large protein comple,x termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation.
Immunogen:    Fusion protein of human ANAPC2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 217013(ANAPC2 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 217013(Anti-ANAPC2 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Hela cells, Jurkat cells, NIH/3T3 cells;
Primary antibody: 217013(ANAPC2 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds
          Publications