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ANAPC5 Rabbit pAb

Anti-ANAPC5 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217015

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217015
Product Name:   Anti-ANAPC5 Rabbit Polyclonal Antibody
Synonyms:   APC5
UNIPROT ID:   Q9UJX4 (Gene Accession – BC006301 )
Background:    This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human ANAPC5
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 217015(ANAPC5 Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 217015(Anti-ANAPC5 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 217015(Anti-ANAPC5 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D221645(Anti-ANAPC5 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: NIH/3T3 cells;
Primary antibody: 217015(ANAPC5 Antibody) at dilution 1/187.5;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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