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ANO1 Rabbit pAb

Anti-ANO1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220122

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220122
Product Name:   Anti-ANO1 Rabbit Polyclonal Antibody
Synonyms:   DOG1; INDMS; TAOS2; ORAOV2; TMEM16A
UNIPROT ID:   Q5XXA6 (Gene Accession – NP_060513 )
Background:    Enables calcium activated cation channel activity; intracellular calcium activated chloride channel activity; and iodide transmembrane transporter activity. Involved in cation transport; inorganic anion transport; and positive regulation of insulin secretion involved in cellular response to glucose stimulus. Located in apical plasma membrane and nucleoplasm.
Immunogen:    Synthetic peptide of human ANO1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220122(ANO1 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220122(Anti-ANO1 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 220122(Anti-ANO1 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with synthetic peptide and then with D260983(Anti-ANO1 Antibody) at dilution 1/50.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HepG2 and PC-3 cell lysates;
Primary antibody: 220122(ANO1 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 45 seconds
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