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AP2A1 Rabbit pAb

Anti-AP2A1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221165

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221165
Product Name:   Anti-AP2A1 Rabbit Polyclonal Antibody
Synonyms:   ADTAA; CLAPA1; AP2-ALPHA
UNIPROT ID:   O95782 (Gene Accession – NP_055018 )
Background:    This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined.
Immunogen:    Synthetic peptide of human AP2A1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221165(AP2A1 Antibody) at a dilution of 1/55(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221165(Anti-AP2A1 Antibody) at dilution 1/55.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: NIH/3T3 and Hela cell lysates;
Primary antibody: 221165(AP2A1 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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