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APAF1 Rabbit pAb

Anti-APAF1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219605

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219605
Product Name:   Anti-APAF1 Rabbit Polyclonal Antibody
Synonyms:   CED4
UNIPROT ID:   O14727 (Gene Accession – NP_863659 )
Background:    This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
Immunogen:    Synthetic peptide of human APAF1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 219605(APAF1 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 219605(Anti-APAF1 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 219605(Anti-APAF1 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with synthetic peptide and then with D260008(Anti-APAF1 Antibody) at dilution 1/20.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela cells, A549 cells;
Primary antibody: 219605(APAF1 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 minutes
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