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APOBEC3D Rabbit pAb

Anti-APOBEC3D Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S214073

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S214073
Product Name:   Anti-APOBEC3D Rabbit Polyclonal Antibody
Synonyms:   A3D; ARP6; APOBEC3E; APOBEC3DE
UNIPROT ID:   Q96AK3 (Gene Accession – NP_689639 )
Background:    This gene is a member of the cytidine deaminase gene family. It is one of a group of related genes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1 and inhibit retroviruses, such as HIV, by deaminating cytosine residues in nascent retroviral cDNA.
Immunogen:    Synthetic peptide of human APOBEC3D
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 214073(APOBEC3D Antibody) at a dilution of 1/80(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the synthetic peptide and then with 214073(Anti-APOBEC3D Antibody) at dilution 1/80.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 214073(Anti-APOBEC3D Antibody) at a dilution of 1/80.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with synthetic peptide and then with D161296(Anti-APOBEC3D Antibody) at dilution 1/80.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 231, HEPG2 and Jurkat cell lysates;
Primary antibody: 214073(APOBEC3D Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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