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ARF6 Rabbit pAb

Anti-ARF6 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219637

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219637
Product Name:   Anti-ARF6 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   P62330 (Gene Accession – NP_001654 )
Background:    This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7.
Immunogen:    Synthetic peptide of human ARF6
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: Oct-50;WB: 500-2000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 219637(ARF6 Antibody) at a dilution of 1/15(Cytoplasm ).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the synthetic peptide and then with 219637(Anti-ARF6 Antibody) at dilution 1/15.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HepG2 cells, 231 cells;
Primary antibody: 219637(ARF6 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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