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ARFGEF2 Rabbit pAb

Anti-ARFGEF2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220309

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220309
Product Name:   Anti-ARFGEF2 Rabbit Polyclonal Antibody
Synonyms:   BIG2; PVNH2; dJ1164I10.1
UNIPROT ID:   Q9Y6D5 (Gene Accession – NP_006411 )
Background:    ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.
Immunogen:    Synthetic peptide of human ARFGEF2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 220309(ARFGEF2 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 220309(Anti-ARFGEF2 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 220309(Anti-ARFGEF2 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D261315(Anti-ARFGEF2 Antibody) at dilution 1/50.

Gel: 6%SDS-PAGE, Lysate: 50 μg;
Lane: Mouse heart tissue;
Primary antibody: 220309(ARFGEF2 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 minutes
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