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ASAH1 Rabbit pAb

Anti-ASAH1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217167

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217167
Product Name:   Anti-ASAH1 Rabbit Polyclonal Antibody
Synonyms:   AC; PHP; ASAH; PHP32; ACDase; SMAPME
UNIPROT ID:   Q13510 (Gene Accession – BC016828 )
Background:    This gene encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease. Multiple transcript variants encoding several distinct isoforms have been identified for this gene.
Immunogen:    Fusion protein of human ASAH1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 217167(ASAH1 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 217167(Anti-ASAH1 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 217167(Anti-ASAH1 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D221909(Anti-ASAH1 Antibody) at dilution 1/40.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Human colon cancer tissue, human fetal brain tissue, Human fetal lung tissue;
Primary antibody: 217167(ASAH1 Antibody) at dilution 1/800;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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