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ASAH2 Rabbit pAb

Anti-ASAH2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217168

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217168
Product Name:   Anti-ASAH2 Rabbit Polyclonal Antibody
Synonyms:   HNAC1; BCDase; LCDase; NCDase; N-CDase
UNIPROT ID:   Q9NR71 (Gene Accession – BC107105 )
Background:    Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.
Immunogen:    Fusion protein of human ASAH2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human brain tissue using 217168(ASAH2 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the fusion protein and then with 217168(Anti-ASAH2 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 217168(Anti-ASAH2 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D221910(Anti-ASAH2 Antibody) at dilution 1/50.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Lovo cells, mouse heart tissue;
Primary antibody: 217168(ASAH2 Antibody) at dilution 1/270;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds
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