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ASPSCR1 Rabbit pAb

Anti-ASPSCR1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219503

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219503
Product Name:   Anti-ASPSCR1 Rabbit Polyclonal Antibody
Synonyms:   TUG; ASPL; ASPS; RCC17; UBXD9; UBXN9; ASPCR1
UNIPROT ID:   Q9BZE9 (Gene Accession – BC018722 )
Background:    The protein encoded by this gene contains a UBX domain and interacts with glucose transporter type 4 (GLUT4). This protein is a tether, which sequesters the GLUT4 in intracellular vesicles in muscle and fat cells in the absence of insulin, and redistributes the GLUT4 to the plasma membrane within minutes of insulin stimulation. Translocation t(X;17)(p11;q25) of this gene with transcription factor TFE3 gene results in a ASPSCR1-TFE3 fusion protein in alveolar soft part sarcoma and in renal cell carcinomas. Multiple alternatively spliced transcript variants have been found.
Immunogen:    Fusion protein of human ASPSCR1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 219503(ASPSCR1 Antibody) at a dilution of 1/80(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 219503(Anti-ASPSCR1 Antibody) at dilution 1/80.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela and 293T cell lysates;
Primary antibody: 219503(ASPSCR1 Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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