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ATG3 Rabbit pAb

Anti-ATG3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217017

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217017
Product Name:   Anti-ATG3 Rabbit Polyclonal Antibody
Synonyms:   APG3; APG3L; hApg3; PC3-96; APG3-LIKE
UNIPROT ID:   Q9NT62 (Gene Accession – BC024221 )
Background:    This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms.
Immunogen:    Fusion protein of human ATG3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Cell Biology, Neuroscience, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 217017(ATG3 Antibody) at a dilution of 1/80(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 217017(Anti-ATG3 Antibody) at dilution 1/80.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 217017(Anti-ATG3 Antibody) at a dilution of 1/80.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D221649(Anti-ATG3 Antibody) at dilution 1/80.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse brain tissue lysate;
Primary antibody: 217017(ATG3 Antibody) at dilution 1/1350;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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