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ATG9A Rabbit pAb

Anti-ATG9A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217148

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217148
Product Name:   Anti-ATG9A Rabbit Polyclonal Antibody
Synonyms:   mATG9; APG9L1; MGD3208
UNIPROT ID:   Q7Z3C6 (Gene Accession – BC001206 )
Background:    Autophagy-related protein 9A is a protein that in humans is encoded by the ATG9A gene. Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes.
Immunogen:    Fusion protein of human ATG9A
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Cell Biology, Neuroscience, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human brain tissue using 217148(ATG9A Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the fusion protein and then with 217148(Anti-ATG9A Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 217148(Anti-ATG9A Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D221877(Anti-ATG9A Antibody) at dilution 1/30.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: 293T cells;
Primary antibody: 217148(ATG9A Antibody) at dilution 1/240;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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