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ATP4B Rabbit pAb

Anti-ATP4B Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216367

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216367
Product Name:   Anti-ATP4B Rabbit Polyclonal Antibody
Synonyms:   ATP6B
UNIPROT ID:   P51164 (Gene Accession – BC029059 )
Background:    The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane.
Immunogen:    Fusion protein of human ATP4B
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216367(ATP4B Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216367(Anti-ATP4B Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 216367(Anti-ATP4B Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D220298(Anti-ATP4B Antibody) at dilution 1/40.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse pancreas tissue;
Primary antibody: 216367(ATP4B Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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