Email: info@neweastbio.com   |  Call: 6109452007

ATP5MPL Rabbit pAb

Anti-ATP5MPL Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221640

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221640
Product Name:   Anti-ATP5MPL Rabbit Polyclonal Antibody
Synonyms:   MLQ; MP68; PLPM; 6.8PL; C14orf2
UNIPROT ID:   P56378 (Gene Accession – NP_004885 )
Background:    Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 – containing the extramembraneous catalytic core and F0 – containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). Minor subunit required to maintain the ATP synthase population in the mitochondria (PubMed:24330338).
Immunogen:    Synthetic peptide of human ATP5MPL
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 40-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 221640(ATP5MPL Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the synthetic peptide and then with 221640(Anti-ATP5MPL Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 221640(Anti-ATP5MPL Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D263326(Anti-ATP5MPL Antibody) at dilution 1/60.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: 293T and RAW264.7 cell?Human fetal brain tissue?Jurkat and 231 cell?Human heart tissue?Hela cell lysates;
Primary antibody: 221640(ATP5MPL Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
          Publications