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ATP6V1E2 Rabbit pAb

Anti-ATP6V1E2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218192

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218192
Product Name:   Anti-ATP6V1E2 Rabbit Polyclonal Antibody
Synonyms:   VMA4; ATP6E1; ATP6EL2; ATP6V1EL2
UNIPROT ID:   Q96A05 (Gene Accession – BC008981 )
Background:    Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. This isoform is essential for energy coupling involved in acidification of acrosome.
Immunogen:    Fusion protein of human ATP6V1E2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 218192(ATP6V1E2 Antibody) at a dilution of 1/120(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 218192(Anti-ATP6V1E2 Antibody) at dilution 1/120.

The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 218192(Anti-ATP6V1E2 Antibody) at a dilution of 1/120.

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with fusion protein and then with D223909(Anti-ATP6V1E2 Antibody) at dilution 1/120.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: TM4 cell, Hela cell, Mouse brain tissue lysates;
Primary antibody: 218192(ATP6V1E2 Antibody) at dilution 1/450;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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