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B2M Rabbit pAb

Anti-B2M Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216354

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216354
Product Name:   Anti-B2M Rabbit Polyclonal Antibody
Synonyms:   IMD43
UNIPROT ID:   P61769 (Gene Accession – BC032589 )
Background:    This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
Immunogen:    Fusion protein of human B2M
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 216354(B2M Antibody) at a dilution of 1/80(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 216354(Anti-B2M Antibody) at dilution 1/80.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: HepG2, Hela, 293T cell lysates;
Primary antibody: 216354(B2M Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 seconds
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