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BAG3 Rabbit pAb

Anti-BAG3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216292

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216292
Product Name:   Anti-BAG3 Rabbit Polyclonal Antibody
Synonyms:   BIS; MFM6; BAG-3; CAIR-1
UNIPROT ID:   O95817 (Gene Accession – BC014656 )
Background:    BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
Immunogen:    Fusion protein of human BAG3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 40-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 216292(BAG3 Antibody) at a dilution of 1/35(Cytoplasm or Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 216292(Anti-BAG3 Antibody) at dilution 1/35.

The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 216292(Anti-BAG3 Antibody) at a dilution of 1/35.

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with fusion protein and then with D220147(Anti-BAG3 Antibody) at dilution 1/35.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse heart tissue lysate;
Primary antibody: 216292(BAG3 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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