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BAP1 Rabbit pAb

Anti-BAP1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217150

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217150
Product Name:   Anti-BAP1 Rabbit Polyclonal Antibody
Synonyms:   UCHL2; hucep-6; HUCEP-13
UNIPROT ID:   Q92560 (Gene Accession – BC001596 )
Background:    This gene belongs to the ubiquitin C-terminal hydrolase subfamily of deubiquitinating enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germline mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, uveal melanoma and cutaneous melanoma.
Immunogen:    Fusion protein of human BAP1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human colorectal cancer tissue using 217150(BAP1 Antibody) at a dilution of 1/70(Nucleus and Cytoplasm).

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: A549, PC-3, Jurkat cell lysates;
Primary antibody: 217150(BAP1 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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