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BBC3 Rabbit pAb

Anti-BBC3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S213393

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S213393
Product Name:   Anti-BBC3 Rabbit Polyclonal Antibody
Synonyms:   JFY1; PUMA; JFY-1
UNIPROT ID:   Q9BXH1 (Gene Accession – NP_055232 )
Background:    This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants.
Immunogen:    Synthetic peptide of human BBC3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 213393(BBC3 Antibody) at a dilution of 1/80(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 213393(Anti-BBC3 Antibody) at dilution 1/80.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 213393(Anti-BBC3 Antibody) at a dilution of 1/80.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D160118(Anti-BBC3 Antibody) at dilution 1/80.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: K562 and 293T cell lysates;
Primary antibody: 213393(BBC3 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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