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BHLHA15 Rabbit pAb

Anti-BHLHA15 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221613

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221613
Product Name:   Anti-BHLHA15 Rabbit Polyclonal Antibody
Synonyms:   MIST1; BHLHB8
UNIPROT ID:   Q7RTS1 (Gene Accession – NP_803238 )
Background:    MIST1 (muscle, intestine and stomach expression 1), also known as BHLHB8 (basic helix-loop-helix domain containing, class B, 8), is a 189 amino acid nuclear protein expressed in liver, brain, skeletal muscle and spleen. MIST1 contains a basic helix-loop-helix (bHLH) domain and belongs to the bHLH family of transcription factors. Members of this family bind to the E-box motifs present in the promoter or enhancer regions of a variety of developmentally regulated genes and function as either transcriptional activators or transcriptional repressors. MIST1 is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins (E12 and E47) and is believed to play a role regulating the transcriptional activity of MyoD, a protein involved in the regulation of muscle cell development. More specifically, MIST1 functions as a repressor of MyoD activity, ensuring that myoblast populations do not differentiate. In addition, MIST1 is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development.
Immunogen:    Synthetic peptide of human BHLHA15
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 20-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 221613(BHLHA15 Antibody) at a dilution of 1/25(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 221613(Anti-BHLHA15 Antibody) at dilution 1/25.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Human muscle tissue, Human spleen tissue, Mouse Pancreas tissue, Human pancreas tissue and Mouse liver tissue lysates;
Primary antibody: 221613(BHLHA15 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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