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BID Rabbit pAb

Anti-BID Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216370

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216370
Product Name:   Anti-BID Rabbit Polyclonal Antibody
Synonyms:   FP497
UNIPROT ID:   P55957 (Gene Accession – BC022072 )
Background:    This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
Immunogen:    Fusion protein of human BID
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-2000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human renal cancer tissue using 216370(BID Antibody) at a dilution of 1/25(Cytoplasm, Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human renal cancer tissue is first treated with the fusion protein and then with 216370(Anti-BID Antibody) at dilution 1/25.

Gel: 12%SDS-PAGE, Lysate: 30 μg;
Lane 1-3: Human fetal brain tissue, HT29 cells, HepG2 cells;
Primary antibody: 216370(BID Antibody) at dilution 1/220;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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