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BTBD6 Rabbit pAb

Anti-BTBD6 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221626

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221626
Product Name:   Anti-BTBD6 Rabbit Polyclonal Antibody
Synonyms:   BDPL
UNIPROT ID:   Q96KE9 (Gene Accession – NP_150374 )
Background:    BTBD6 (BTB/POZ domain-containing protein 6), also known as BDPL or lens BTB domain protein, is a 410 amino acid protein that contains one BTB/POZ domain and is specifically expressed in the lens. The BTB/POZ domain mediates homomeric and heteromeric POZ-POZ interactions and is common to transcriptional regulators involved in chromatin modeling. In several BTB/POZ containing proteins, including BCL-6 and the promyelocytic leukemia zinc-finger (PLZF) oncoprotein, this domain interacts with the SMRT/N-CoR-mSin3A HDAC complex and is directly involved in repressing and silencing gene transcription. When this domain is deleted, as with the oncogenic PLZF-RAR chimera of promyelocytic leukemias, this transcriptional repression is attenuated. This suggests that BTBD6 may play a role in transcription regulation in the lens.
Immunogen:    Synthetic peptide of human BTBD6
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: Oct-50;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 221626(BTBD6 Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 221626(Anti-BTBD6 Antibody) at dilution 1/25.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: Human gastric carcinoma tissue lysate;
Primary antibody: 221626(BTBD6 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 minutes
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