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CAPN1 Rabbit pAb

Anti-CAPN1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216380

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216380
Product Name:   Anti-CAPN1 Rabbit Polyclonal Antibody
Synonyms:   CANP; muCL; CANP1; SPG76; CANPL1; muCANP
UNIPROT ID:   P07384 (Gene Accession – BC017200 )
Background:    The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.
Immunogen:    Fusion protein of human CAPN1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 216380(CAPN1 Antibody) at a dilution of 1/75(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 216380(Anti-CAPN1 Antibody) at dilution 1/75.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 216380(Anti-CAPN1 Antibody) at a dilution of 1/75.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D220328(Anti-CAPN1 Antibody) at dilution 1/75.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: K562, Jurkat and Hela cell lysates;
Primary antibody: 216380(CAPN1 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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