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CAPN14 Rabbit pAb

Anti-CAPN14 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221669

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221669
Product Name:   Anti-CAPN14 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   A8MX76 (Gene Accession – NP_001138594 )
Background:    Calpains are a family of cytosolic calcium-activated cysteine proteases involved in a variety of cellular processes including apoptosis, cell division, modulation of integrin-cytoskeletal interactions, and synaptic plasticity (Dear et al., 2000 [PubMed 10964513]). CAPN14 belongs to the calpain large subunit family.
Immunogen:    Synthetic peptide of human CAPN14
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 221669(CAPN14 Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 221669(Anti-CAPN14 Antibody) at dilution 1/60.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: Human cerebrum tissue lysate;
Primary antibody: 221669(CAPN14 Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 15 seconds
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