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CARD16 Rabbit pAb

Anti-CARD16 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221761

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221761
Product Name:   Anti-CARD16 Rabbit Polyclonal Antibody
Synonyms:   COP; COP1; PSEUDO-ICE
UNIPROT ID:   Q5EG05 (Gene Accession – NP_001017534 )
Background:    Caspase inhibitor. Acts as a regulator of procaspase-1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro-inflammatory cytokine response. Also able to inhibit CASP1-mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, and staurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspases CASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2.
Immunogen:    Synthetic peptide of human CARD16
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221761(CARD16 Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221761(Anti-CARD16 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 221761(Anti-CARD16 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D263494(Anti-CARD16 Antibody) at dilution 1/25.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: HEPG2 cell lysate;
Primary antibody: 221761(CARD16 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 60 seconds
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