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CASP8 Rabbit pAb

Anti-CASP8 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222437

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S222437
Product Name:   Anti-CASP8 Rabbit Polyclonal Antibody
Synonyms:   CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
UNIPROT ID:   Q14790 (Gene Accession – NP_203519 )
Background:    This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
Immunogen:    Synthetic peptide of human CASP8
Applications:    ELISA, IHC
Recommended Dilutions:    IHC: 50-200; ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 222437(CASP8 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 222437(Anti-CASP8 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 222437(Anti-CASP8 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with synthetic peptide and then with D264607(Anti-CASP8 Antibody) at dilution 1/50.
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