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CCNJ Rabbit pAb

Anti-CCNJ Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S215609

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S215609
Product Name:   Anti-CCNJ Rabbit Polyclonal Antibody
Synonyms:   bA690P14.1
UNIPROT ID:   Q5T5M9 (Gene Accession – NP_061957 )
Background:    Cyclin J is a 372 amino acid protein encoded by the human gene CCNJ located on chromosome 10. Cyclin J belongs to the cyclin family and contains one cyclin N-terminal domain. A cyclin forms a complex with its partner cyclin-dependent kinase (Cdk), which activates the protein kinase function of the Cdk. Cyclins are so named because their concentration varies in a cyclical fashion during the cell cycle. They are produced or degraded as needed in order to drive the cell through the different stages of the cell cycle. When its concentrations in the cell are low, the cyclin detaches from the Cdk, inhibiting the activity of the enzyme, probably by causing a protein chain to block the enzymatic site. Cyclin J-associated kinase activity is required for the early embryonic division cycles.
Immunogen:    Synthetic peptide of human CCNJ
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: Oct-50;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 215609(CCNJ Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the synthetic peptide and then with 215609(Anti-CCNJ Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: K562 cell lysate;
Primary antibody: 215609(CCNJ Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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