Email: info@neweastbio.com   |  Call: 6109452007

CD247 Rabbit pAb

Anti-CD247 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218446

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218446
Product Name:   Anti-CD247 Rabbit Polyclonal Antibody
Synonyms:   T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3-ZETA
UNIPROT ID:   P20963 (Gene Accession – BC025703 )
Background:    The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Immunogen:    Fusion protein of human CD247
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Immunology, Stem Cells
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 218446(CD247 Antibody) at a dilution of 1/30(Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 218446(Anti-CD247 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 218446(Anti-CD247 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D224458(Anti-CD247 Antibody) at dilution 1/30.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Jurkat cell lysate;
Primary antibody: 218446(CD247 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
          Publications