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CD33 Rabbit pAb

Anti-CD33 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217265

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217265
Product Name:   Anti-CD33 Rabbit Polyclonal Antibody
Synonyms:   p67; SIGLEC3; SIGLEC-3
UNIPROT ID:   P20138 (Gene Accession – BC028152 )
Background:    Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
Immunogen:    Fusion protein of human CD33
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Immunology, Stem Cells
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 217265(CD33 Antibody) at a dilution of 1/30(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 217265(Anti-CD33 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: HepG2 cell, Mouse liver tissue, Jurkat cell lysates;
Primary antibody: 217265(CD33 Antibody) at dilution 1/750;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 minutes
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