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CD47 Rabbit pAb

Anti-CD47 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220442

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220442
Product Name:   Anti-CD47 Rabbit Polyclonal Antibody
Synonyms:   IAP; OA3; MER6
UNIPROT ID:   Q08722 (Gene Accession – NP_001768 )
Background:    This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene.
Immunogen:    Synthetic peptide of human CD47
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human brain tissue using 220442(CD47 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the synthetic peptide and then with 220442(Anti-CD47 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human lymphoma tissue using 220442(Anti-CD47 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human lymphoma tissue is first treated with synthetic peptide and then with D261534(Anti-CD47 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Human metastatic poorly differentiated adenocarcinoma of the ovary tissue, Human ovarian cancer tissue lysate, Human ovarian cancer ( granulosa cell tumor ) tissue lysates;
Primary antibody: 220442(CD47 Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 minutes
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