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CD99 Rabbit pAb

Anti-CD99 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218450

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218450
Product Name:   Anti-CD99 Rabbit Polyclonal Antibody
Synonyms:   MIC2; HBA71; MIC2X; MIC2Y; MSK5X
UNIPROT ID:   P14209 (Gene Accession – BC002584 )
Background:    The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus.
Immunogen:    Fusion protein of human CD99
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 218450(CD99 Antibody) at a dilution of 1/25(Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 218450(Anti-CD99 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 218450(Anti-CD99 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with fusion protein and then with D224479(Anti-CD99 Antibody) at dilution 1/25.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Human spleen tissue, K562 cell, Human left thymus tissue lysates;
Primary antibody: 218450(CD99 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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