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CDC20 Rabbit pAb

Anti-CDC20 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216405

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216405
Product Name:   Anti-CDC20 Rabbit Polyclonal Antibody
Synonyms:   CDC20A; p55CDC; bA276H19.3
UNIPROT ID:   Q12834 (Gene Accession – BC010044 )
Background:    CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation.
Immunogen:    Fusion protein of human CDC20
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 200-400;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 216405(CDC20 Antibody) at a dilution of 1/200(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 216405(Anti-CDC20 Antibody) at dilution 1/200.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 216405(Anti-CDC20 Antibody) at a dilution of 1/200.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with fusion protein and then with D220392(Anti-CDC20 Antibody) at dilution 1/200.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: SKOV3 and Hela cell lysates;
Primary antibody: 216405(CDC20 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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