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CDC23 Rabbit pAb

Anti-CDC23 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218453

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218453
Product Name:   Anti-CDC23 Rabbit Polyclonal Antibody
Synonyms:   APC8; CUT23; ANAPC8
UNIPROT ID:   Q9UJX2 (Gene Accession – BC017713 )
Background:    The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction.
Immunogen:    Fusion protein of human CDC23
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 20-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 218453(CDC23 Antibody) at a dilution of 1/20(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 218453(Anti-CDC23 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 218453(Anti-CDC23 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D224484(Anti-CDC23 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 293T, SKOV3 and Hela cell lysates;
Primary antibody: 218453(CDC23 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds
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