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CDC27 Rabbit pAb

Anti-CDC27 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216406

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216406
Product Name:   Anti-CDC27 Rabbit Polyclonal Antibody
Synonyms:   APC3; HNUC; NUC2; ANAPC3; CDC27Hs; D0S1430E; D17S978E
UNIPROT ID:   P30260 (Gene Accession – BC011656 )
Background:    The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human CDC27
Applications:    ELISA, IHC
Recommended Dilutions:    IHC: 50-200; ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 216406(CDC27 Antibody) at a dilution of 1/50(Nucleus ).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 216406(Anti-CDC27 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 216406(Anti-CDC27 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with fusion protein and then with D220393(Anti-CDC27 Antibody) at dilution 1/50.
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