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CDC34 Rabbit pAb

Anti-CDC34 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220449

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220449
Product Name:   Anti-CDC34 Rabbit Polyclonal Antibody
Synonyms:   UBC3; UBCH3; UBE2R1; E2-CDC34
UNIPROT ID:   P49427 (Gene Accession – NP_004350 )
Background:    The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein comple,x which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication.
Immunogen:    Synthetic peptide of human CDC34
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human Lymphoma tissue using 220449(CDC34 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human Lymphoma tissue is first treated with the synthetic peptide and then with 220449(Anti-CDC34 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 220449(Anti-CDC34 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D261544(Anti-CDC34 Antibody) at dilution 1/50.

Gel: 15%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela cells, A431 cells;
Primary antibody: 220449(CDC34 Antibody) at dilution 1/900;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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