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CDC42BPA Rabbit pAb

Anti-CDC42BPA Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221705

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221705
Product Name:   Anti-CDC42BPA Rabbit Polyclonal Antibody
Synonyms:   MRCK; MRCKA; PK428
UNIPROT ID:   Q5VT25 (Gene Accession – XP_005273381 )
Background:    The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported.
Immunogen:    Synthetic peptide of human CDC42BPA
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221705(CDC42BPA Antibody) at a dilution of 1/45(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221705(Anti-CDC42BPA Antibody) at dilution 1/45.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: A172 cell lysate;
Primary antibody: 221705(CDC42BPA Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 minutes
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