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CDKL4 Rabbit pAb

Anti-CDKL4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221709

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221709
Product Name:   Anti-CDKL4 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   Q5MAI5 (Gene Accession – NP_001009565 )
Background:    Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Cdks are considered potential targets for anti-cancer therapy due to their involvement with cell cycle regulation. Cdks are also involved in the regulation of transcription and mRNA processing. CdkL4 (cyclin-dependent kinase-like 4) is a 315 amino acid protein that belongs to the CMGC Ser/Thr protein kinase family and may be involved in cell cycle regulation.
Immunogen:    Synthetic peptide of human CDKL4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221709(CDKL4 Antibody) at a dilution of 1/35(Cytoplasm or Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221709(Anti-CDKL4 Antibody) at dilution 1/35.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 221709(Anti-CDKL4 Antibody) at a dilution of 1/35.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D263418(Anti-CDKL4 Antibody) at dilution 1/35.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human heart tissue lysate;
Primary antibody: 221709(CDKL4 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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