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CDKN1B Rabbit pAb

Anti-CDKN1B Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216415

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216415
Product Name:   Anti-CDKN1B Rabbit Polyclonal Antibody
Synonyms:   KIP1; MEN4; CDKN4; MEN1B; P27KIP1
UNIPROT ID:   P46527 (Gene Accession – BC001971 )
Background:    This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4).
Immunogen:    Fusion protein of human CDKN1B
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 216415(CDKN1B Antibody) at a dilution of 1/50(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 216415(Anti-CDKN1B Antibody) at dilution 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Jurkat and Raji cell lysates;
Primary antibody: 216415(CDKN1B Antibody) at dilution 1/400;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 seconds
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