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CKAP4 Rabbit pAb

Anti-CKAP4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220461

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220461
Product Name:   Anti-CKAP4 Rabbit Polyclonal Antibody
Synonyms:   p63; CLIMP-63; ERGIC-63
UNIPROT ID:   Q07065 (Gene Accession – NP_006816 )
Background:    CKAP4 (cytoskeleton-associated protein 4), also known as p63, CLIMP-63 or ERGIC-63, is a 602 amino acid single-pass type II transmembrane protein that links the endoplasmic reticulum (ER) to the cytoskeleton. Considered a novel protein in maintaining ER morphology, CKAP4 anchors the ER to microtubles which is required for maintaining ER spatial distribution during interphase of the cell cycle. CKAP4 can be reversibly palmitoylated and phosphorylated and is a major substrate of the palmitoyl acyltransferase, ZDHHC2.
Immunogen:    Synthetic peptide of human CKAP4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 1000-5000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 220461(CKAP4 Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the synthetic peptide and then with 220461(Anti-CKAP4 Antibody) at dilution 1/30.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 293T cells, hepG2 cells, A549 cells;
Primary antibody: 220461(CKAP4 Antibody) at dilution 1/750;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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