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CLDN1 Rabbit pAb

Anti-CLDN1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219808

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219808
Product Name:   Anti-CLDN1 Rabbit Polyclonal Antibody
Synonyms:   CLD1, SEMP1, ILVASC
UNIPROT ID:   O95832 (Gene Accession – NP_066924 )
Background:    Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.
Immunogen:    Synthetic peptide of human CLDN1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 2000-5000;ELISA: 1000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 219808(CLDN1 Antibody) at a dilution of 1/150(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 219808(Anti-CLDN1 Antibody) at dilution 1/150.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 219808(Anti-CLDN1 Antibody) at a dilution of 1/150.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D260403(Anti-CLDN1 Antibody) at dilution 1/150.

Gel: 10%SDS-PAGE, Lysate: 30 μg;
Lane: Hela cells;
Primary antibody: 219808(CLDN1 Antibody) at dilution 1/1900;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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