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Cleaved-Caspase 4/5 p20 Rabbit pAb

Anti-Cleaved-Caspase 4/5 p20 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225494

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225494
Product Name:   Anti-Cleaved-Caspase 4/5 p20 Rabbit pAb
Synonyms:   TX; Mih1; ICH-2; Mih1/TX; ICEREL-II; ICE(rel)II
UNIPROT ID:   P49662/P51878
Background:    CASP4 (caspase 4) encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, caspase 4 induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.
Immunogen:    The antiserum was produced against synthesized peptide derived from human Caspase 4/5. AA range:221-270
Applications:    WB,ELISA
Recommended Dilutions:    WB: 1/500-1/1000 ELISA: 1/10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 43 kDa; Observed MW: 47,22 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Cleaved
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    apoptosis – Caspase family
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of Cleaved-Caspase 4/5 p20 in Jurkat lysates using Caspase 4/5 (p20, Cleaved-Asp270/Asp311) antibody. The lane on the right is blocked with the Caspase 4/5 (p20, Cleaved-Asp270/Asp311) peptide.
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