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CLTC Rabbit pAb

Anti-CLTC Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220462

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220462
Product Name:   Anti-CLTC Rabbit Polyclonal Antibody
Synonyms:   Hc; CHC; CHC17; CLH-17; CLTCL2
UNIPROT ID:   Q00610 (Gene Accession – NP_004850 )
Background:    Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.
Immunogen:    Synthetic peptide of human CLTC
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gasrtic cancer tissue using 220462(CLTC Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human gasrtic cancer tissue is first treated with the synthetic peptide and then with 220462(Anti-CLTC Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 220462(Anti-CLTC Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D261565(Anti-CLTC Antibody) at dilution 1/20.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A172 cells, hela cells;
Primary antibody: 220462(CLTC Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds
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