Email: info@neweastbio.com   |  Call: 6109452007

Cofilin Rabbit pAb

Anti-Cofilin Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225279

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225279
Product Name:   Anti-Cofilin Rabbit pAb
Synonyms:   CFL1; CFL; Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin; non-muscle isoform
UNIPROT ID:   P23528
Background:    The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]
Immunogen:    Peptide sequence around aa. 86~90 (A-T-Y-E-T) derived from Human coflin1/cofilin2.
Applications:    WB,IHC-P,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 19 kDa; Observed MW: 19 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunofluorescence analysis of Cofilin in Hela using coflin1/cofilin2 (Ab88) antibody.

Western blot analysis of Cofilin in MDA lysates using coflin1/cofilin2 (Ab88) antibody.

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using coflin1/cofilin2 (Ab88) antibody (left) or the same antibody preincubated with blocking peptide (right).High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
          Publications